My name is Ilya Avros, and I’m a rising 3rd-year mechanical engineering major at Macaulay Honors College at the City College of New York. My project this summer is a Dropbased Microfluidic Approach to Protein Crystallization. This project involves the manufacturing of PDMS-based microfluidic chips using soft lithography. Each chip is tested using HFE oil as the continuous phase, and NaCl, Lysozyme, and NaAc as the dispersed phase, with flow rates and concentrations being controlled experimentally. The goal of this project is to grow larger and purer protein crystals that are necessary to treat various illnesses. This approach to protein crystallization offers precise control and higher yields over its batch-based counterpart, significantly reducing the waste of expensive materials.

Click Here for PDF Presentation